The purpose of this database was to explore the prediction of new cultivation-independent organism-media based on probiotic culture media database (DPCM). In this study, a probiotic medium database was established by collecting existing media formulations from resources such as ATCC. Thereafter, the strain was isolated and purified, and its genome was extracted. Then PCR amplification and 16S rDNA sequencing were performed. The 16S rDNA sequence of this strain was analyzed to construct a database. Several unknown microorganism culture media have been predicted by the above method. Through many experiments, the potential application value of the database has been confirmed.