Probiotics are a group of active microorganisms beneficial to the host, which are planted in the human intestinal reproductive system and can produce precise health effects to improve the microecological balance of the host. The general term of active beneficial microorganisms mainly includes: lactic acid bacteria, bifidobacteria, lactobacillus acidophilus, actinomycetes, yeast and so on. At present, probiotics are widely used in various fields and play an important role in ensuring the health of humans and animals or preventing and treating certain diseases. With the deepening of the research on probiotics nutritional factors, promoting the growth of various probiotics and preventing probiotics reduce the number of substances that lead to related diseases gradually increased, such as dietary fiber in vegetables. In order to integrate the data resources and results of numerous studies and promote comparative studies, literature review and information extraction analysis were carried out, thereby constructing a knowledge base of probiotic nutritional factors to assist research in promoting the growth of probiotics and treating related diseases, covering source, classification, name, composition & content, molecular weight, molecular formula, CAS number, target, function, other efficacy , references and corresponding links of probiotic nutritional factors. This interface supports the query function for the content of the above probiotic nutritional factors. Therefore, this database will contribute to further research on factors that promote probiotic growth and the treatment of related diseases caused by probiotic reduction, and promote screening strategies for probiotic nutritional factors.