Serofluid dish is one of the most popular probiotic fermented foods in northern China with a long history. It has not only unique flavor, but also rich nutrition, which is beneficial to human health. There are a lot of probiotics resources in Serofluid dish, such as Lactobacillus and Acetobacter are its dominant species. The lactic acid bacteria contained in it can promote gastrointestinal peristalsis, digestion and absorption after entering the human digestive tract; it also can reduce cholesterol and enhance the body's immunity. In Lanzhou, it is not only a local specialty representative of the region, but also a symbol of the local food culture, attracting a large number of domestic food lovers. Based on the systematic study of the microbial community structure of Serofluid dish, the separation and identification results of natural fermentation Serofluid dish samples collected from different geographical locations were summarized, and the database was compiled. The main contents of the database include: species and genera of culturable microorganisms isolated from Serofluid dish, and GenBank accession, corresponding media and other information.